Next Generation Sequencing and Arrays

The NovaSeq X Plus (NovaSeq X+) is Illumina’s highest output sequencing instrument. The NovaSeq X+ uses XLEAP-SBS, offering 3X higher accuracy over their standard sequencing by synthesis (SBS) method. With a variety of flow cells available, 1.5B, 5B, 10B and 25B, and lane configurations, the NovaSeq X+ offers up to 21Tb of data and 25-30B reads per run at maximum capacity. 

Flow Cell1.5B5B10B25B
Flow Cells processed1 or 21 or 21 or 21 or 2
Single end reads1.6-2.4B5B10-13B26-35B
Max read length2 X 1502 X 1502 X 1502 X 150

The Illumina MiSeq (the CGI has two MiSeqs) is designed for rapid sequencing, offering high-accuracy, short-read sequencing with flow cell options of varying read lengths and outputs (1M-25M single end reads, 2M-50M paired end reads). This platform is optimal for screening pools of libraries, small genome sequencing, amplicon sequencing (including 16S) and targeted resequencing.

Flow CellV3V2MicroNano
Output range3.8-15Gb0.6-8.5Gb1.2Gb500mb
Single end reads25M12-15M4M1M
Max read length2 X 3002 X 2502 X 2502 X 250

The Illumina NextSeq 2000, uses XLEAP-SBS chemistry and offers more versatility than the NextSeq 550, with 4 flow cells and 8 different read configurations. rapid sequencing, offering high-accuracy, short-read sequencing, with two flow cell options.

Flow CellP1P2P3P4
Output range10-60Gb40-240Gb120-360Gb90-450Gb
Single end/PE reads100M/200M400M/800M1.2B/2.4B1.8B/3.6B
Max read length2 X 50
2 X 100
2 X 300
2 X 50
2 X 100
2 X 150
2 X 300
2 X 50
2 X 100
2 X 150
1 X 50
2 X 50
2 X 100
2 X 150

The Illumina NextSeq 550 offers a high resolution imaging platform used to scan Illumina BeadChip arrays for applications such as SNP genotyping and DNA methylation profiling.

Offering more data than the PacBio Sequel Iie, our PacBio Revio is a long-read sequencing platform utilizing SMRT technology to generate highly accurate HiFi reads (90% Q30+, up to 20 kb). Applications include de novo genome assembly, amplicon sequencing, metagenomes, viral and microbial genomes, structural variant detection, epigenomics and full-length RNA sequencing. On this instrument, 4 SMRT Cells are on a single tray and can be run simultaneously with SPRQ chemistry.

Each SMRT Cell 125M contains ~25 million zero-mode waveguides (ZMWs), yielding up to 120 Gb of data per flow cell. 

NEW CHEMISTRY RELEASE (MAY 2026)

SPRQ-Nx chemistry (featuring multi-use SMRT Cells), which launched globally in May 2026, reduces per-SMRT cell costs while introducing multi-use SMRT Cell reuse and advanced AI consensus algorithms

  • Lower costs: Allows individual SMRT Cells to be washed and automatically reused for multiple sample sets on the Revio deck without manual user intervention, reducing the per SMRT cell run cost by 30-40%.
  • AI Enhancements: Integrates upgraded DeepConsensus algorithms optimized with Google’s AlphaEvolve coding agent for faster processing and higher accuracy. 
    Expanded Epigenetics: Natively supports improved on-instrument calling for 5mC and 6mA, alongside a brand-new 5-hydroxymethylcytosine (5hmC) detector in every run. 

Our PacBio Sequel IIe is no longer used as it has been superseded by the PacBio Revio. If your application requires the Sequel IIe, please contact us.

Our Promethion P24 is an ultra-high-throughput benchtop sequencing device by Oxford Nanopore Technologies featuring 24 individually addressable flow cells, real-time long-read DNA/RNA capability, and a total device output capacity up to 4.8 terabases.

  • Flow cells: Up to 24 independent, simultaneously or separately run positions
  • Typical output: 100–200 Gb per flow cell (yielding 2.4–4.8 Tb per full device run capacity)
  • Read length: Ranging from 20 bp to over 4 [Mb]
  • Run time: Flexible configurations from under 1 hour up to 72 hours per run
  • Compute support: Powered by an integrated Data Acquisition Unit with high-RAM architecture and GPU acceleration for real-time base calling
  • Epigenomics: Supports identification of native DNA and RNA base modifications at single-nucleotide resolution, including m6A in RNA, and 5mC, 5hmC, and 6mA in DNA

Applications supported include: large genomes, population-scale sequencing, highly multiplexed small genomes or targeted regions, long read and ultra-long read sequencing (WGS), adaptive sequencing, metagenome assembly, isoform-level whole transcriptomes (direct RNA, cDNA), epigenomics, modified applications such as Fiber-seq and DiMeLo-seq, and amplicon sequencing.

Our PromethION P2i is capable of running 2 flow cells at one time and is supported by high-performance onboard compute for real-time sequencing and analysis. 

The P2i supports the sample applications as the P24 and provides:

  • Flow cells: Up to 2 independent, simultaneously or separately run positions
  • Typical output: 100–200 Gb per flow cell (yielding 2.4–4.8 Tb per full device run capacity)
  • Read length: Ranging from 20 bp to over 4 [Mb]
  • Run time: Flexible configurations from under 1 hour up to 72 hours per run
  • Compute support: Incorporates a powerful NVIDIA A-Series GPU on board
  • Epigenomics: Supports identification of native DNA and RNA base modifications at single-nucleotide resolution, including m6A in RNA, and 5mC, 5hmC, and 6mA in DNA

Our ONT GridION supports running up to 5 independent MinION flow cells simultaneously. MinION flow cells are Ideal for bacterial isolates, bacterial and viral whole genome sequencing, amplicon (metagenomic or targeted site), whole exome, and smaller transcriptomes.

  • Flow cells: Up to 5 independent, simultaneously or separately run positions
  • Typical output: 15-35 Gb per flow cell
  • Read length: Ranging from 20 bp to over 4 [Mb]
  • Run time: Flexible configurations from under 1 hour up to 72 hours per run
  • Epigenomics: Supports identification of native DNA and RNA base modifications at single-nucleotide resolution, including m6A in RNA, and 5mC, 5hmC, and 6mA in DNA

We have several ONT MinIONs, palm-sized, portable sequencers, each capable of running a single MinION flow cell when combined with a user laptop.

  • Flow cells: 1 flow cell capacity
  • Typical output: 15-35 Gb per flow cell
  • Read length: Ranging from 20 bp to over 4 [Mb]
  • Run time: Flexible configurations from under 1 hour up to 72 hours per run